It has been challenging to meet all of these criteria 11. When scaling to simultaneous multi-well longitudinal tissue imaging it is also important that the apparatus not be bulky or expensive. The resolution of the microscope should be sufficient to view the phenomenon being studied. The microscope should be capable of imaging in the environmental conditions needed for the desired experiment, including temperature, light, and humidity. The microscope should be able to acquire images without damaging or disturbing the specimen, such as photobleaching. The image acquisition speed of the microscope should be sufficient for the phenomenon being studied. When deciding between the different technologies for longitudinal live tissue imaging, several factors need to be considered in the experimental design. These range from super-resolution microscopes, that allow the imaging of individual biomolecules 2, 3, to conventional benchtop microscopes, which are common in academic research 3, 4, 5, 6, 7, industrial 8, 9, and teaching laboratories 10. Several options exist on the market to perform longitudinal imaging of biological materials. Imaging has been pivotal to uncovering cellular mechanisms behind biological processes 1. Advances in microscopy have revolutionized biological studies, allowing scientists to perform observations of cellular processes and organisms’ development and behaviors. Monitoring and handling live tissues and cell cultures as well as analyzing their secreted contents are essential tasks in experimental biology and biomedicine. Using this tool, we can measure the behavior of entire organisms or individual cells over long-time periods. We also gathered image data inside an incubator to observe 2D monolayers and 3D mammalian tissue culture models. We gathered longitudinal whole organism image data for frogs, zebrafish, and planaria worms. Here, we use this system in a range of applications. The Picroscope can be controlled remotely, allowing for automatic imaging with minimal intervention from the investigator. The Picroscope is compatible with standard 24-well cell culture plates and captures 3D z-stack image data. Here, we propose the Picroscope, which is the first low-cost system for simultaneous longitudinal biological imaging made primarily using off-the-shelf and 3D-printed materials. Yet, imaging systems capable of accomplishing these tasks are economically unattainable for most academic and teaching laboratories around the world. Simultaneous longitudinal imaging across multiple conditions and replicates has been crucial for scientific studies aiming to understand biological processes and disease.
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